The dioxin regulation criteria in the agro-industrial complex of Russia

Abstract

The article studies the reactions of biochemical, hematological, pathomorphological, immunological and ultrastructural changes in the organisms of laboratory animals that were subjected to chronic dioxin poisoning at threshold doses were studied. There were hold toxicological experiments on white rats of both sexes weighing 155–215 g in order to detect threshold doses. For two months, 2,3,7,8-tetrachlorodibenzo-para-dioxin (2,3,7,8- TCDD) in amounts corresponding to 1/100 and 1/200 LD 50. After each decade, some of the animals were euthanized and weighed to calculate the mass coefficient of internal organs. The obtained data comparison revealed no statistically significant changes in organ. Continued observation of animals in the experiment for up to two months, characteristic intoxication 2,3,7,8-TCDD clinical signs did not give. The correlation dependence of the minimum effective doses of 2,3,7,8-TCDD, namely 1/400 of LD50 with LD50, was determined. The obtained threshold dose of dioxin can be recommended as the basis for the normalization of dioxin in the Russian agro-industrial complex. There were determined biochemical, hematological and ultrastructural changes in the body of animals exposed to chronic poisoning of 2,3,7,8-TCDD in doses of 1/200, 1/300 of LD50. The article describes a statistically significant dependence of the respiratory activity of the liver mitochondria in laboratory animals (white rats and rabbits) on chronic poisoning with threshold doses of various degrees. Based on laboratory animals’ experiments, threshold concentrations are determined and the maximum permissible levels of dioxin in the feed of some animals are tentatively calculated.