<em>Staphylococcus ratti</em> sp. nov. Isolated from a Lab Rat
Vojtěch Kovařovic,
Ivo Sedláček,
Petr Petráš,
Stanislava Králová,
Ivana Mašlaňová,
Pavel Švec,
Meina Neumann-Schaal,
Tibor Botka,
Tereza Gelbíčová,
Eva Staňková,
Jiří Doškař,
Roman Pantůček
Affiliations
Vojtěch Kovařovic
Department of Experimental Biology, Division of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
Ivo Sedláček
Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
Petr Petráš
Reference Laboratory for Staphylococci, National Institute of Public Health, Šrobárova 48, 100 42 Praha, Czech Republic
Stanislava Králová
Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
Ivana Mašlaňová
Department of Experimental Biology, Division of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
Pavel Švec
Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
Meina Neumann-Schaal
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7 B, 38124 Braunschweig, Germany
Tibor Botka
Department of Experimental Biology, Division of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
Tereza Gelbíčová
Department of Public Health, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
Eva Staňková
Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
Jiří Doškař
Department of Experimental Biology, Division of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
Roman Pantůček
Department of Experimental Biology, Division of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
Staphylococci from the Staphylococcus intermedius-Staphylococcus hyicus species group include numerous animal pathogens and are an important reservoir of virulence and antimicrobial resistance determinants. Due to their pathogenic potential, they are possible causative agents of zoonoses in humans; therefore, it is important to address the properties of these strains. Here we used a polyphasic taxonomic approach to characterize the coagulase-negative staphylococcal strain NRL/St 03/464T, isolated from the nostrils of a healthy laboratory rat during a microbiological screening of laboratory animals. The 16S rRNA sequence, MALDI-TOF mass spectrometry and positive urea hydrolysis and beta-glucuronidase tests clearly distinguished it from closely related Staphylococcus spp. All analyses have consistently shown that the closest relative is Staphylococcus chromogenes; however, values of digital DNA-DNA hybridization Staphylococcus species. Whole-genome sequencing and expert annotation of the genome revealed the presence of novel variable genetic elements, including two plasmids named pSR9025A and pSR9025B, prophages, genomic islands and a composite transposon that may confer selective advantages to other bacteria and enhance their survival. Based on phenotypic, phylogenetic and genomic data obtained in this study, the strain NRL/St 03/464T (= CCM 9025T = LMG 31873T = DSM 111348T) represents a novel species with the suggested name Staphylococcus ratti sp. nov.