Human umbilical cord blood stem cells upregulate matrix metalloproteinase-2 in rats after spinal cord injury

Krishna Kumar Veeravalli; Venkata Ramesh Dasari; Andrew J. Tsung; Dzung H. Dinh; Meena Gujrati; Dan Fassett; Jasti S. Rao

Neurobiology of Disease (Oct 2009)

Human umbilical cord blood stem cells upregulate matrix metalloproteinase-2 in rats after spinal cord injury

Krishna Kumar Veeravalli,
Venkata Ramesh Dasari,
Andrew J. Tsung,
Dzung H. Dinh,
Meena Gujrati,
Dan Fassett,
Jasti S. Rao

Affiliations

Krishna Kumar Veeravalli: Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
Venkata Ramesh Dasari: Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
Andrew J. Tsung: Department of Neurosurgery, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
Dzung H. Dinh: Department of Neurosurgery, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
Meena Gujrati: Department of Pathology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
Dan Fassett: Department of Neurosurgery, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA
Jasti S. Rao: Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA; Department of Neurosurgery, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA; Corresponding author. Department of Cancer Biology and Pharmacology and Department of Neurosurgery, University of Illinois College of Medicine at Peoria, One Illini Drive, Peoria, IL 61605, USA. Fax: +1 309 671 3442.

Journal volume & issue: Vol. 36, no. 1
pp. 200 – 212

Abstract

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Matrix metalloproteinases (MMPs) are a large family of proteolytic enzymes involved in inflammation, wound healing and other pathological processes after neurological disorders. MMP-2 promotes functional recovery after spinal cord injury (SCI) by regulating the formation of a glial scar. In the present study, we aimed to investigate the expression and/or activity of several MMPs, after SCI and human umbilical cord blood mesenchymal stem cell (hUCB) treatment in rats with a special emphasis on MMP-2. Treatment with hUCB after SCI altered the expression of several MMPs in rats. MMP-2 is upregulated after hUCB treatment in spinal cord injured rats and in spinal neurons injured either with staurosporine or hydrogen peroxide. Further, hUCB induced upregulation of MMP-2 reduced formation of the glial scar at the site of injury along with reduced immunoreactivity to chondroitin sulfate proteoglycans. Blockade of MMP-2 activity in hUCB cocultured injured spinal neurons reduced the protection offered by hUCB which indicated the involvement of MMP-2 in the neuroprotection offered by hUCB. Based on these results, we conclude that hUCB treatment after SCI upregulates MMP-2 levels and reduces the formation of the glial scar thereby creating an environment suitable for endogenous repair mechanisms.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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