Correlation of NPRL2 with cinicopathological features of bladder cancer and its effect on cell apoptosis

Abstract

Objective To investigate the correlation between expression of NPRL2 and clinicopathological features of bladder cancer and the effect of NPRL2 silencing on the apoptosis of bladder cancer cell line Biu87/RT-4. Methods Cancer tissue samples were collected from 131 patients with bladder cancer admitted into our department from January 2014 to January 2019. The expression of NPRL2 in bladder cancer tissues was detected by immunohistochemistry, and the correlation between NPRL2 and the clinicopathological features of bladder cancer was analyzed. The expression of NPRL2 in CP-H068, Biu87 and RT-4 cell lines was detected, and the cell proliferation after NPRL2 silencing was detected by CCK-8 assay. Flow cytometry was used to detect cell apoptosis, and Western blotting to detect the expression of apoptosis-related proteins. Results NPRL2 was highly expressed in bladder cancer samples, accounting for 53.44%, and the expression was significantly correlated with the clinical stage and grade of bladder cancer (P < 0.05). Its expression was also obviously higher in the bladder cancer Biu87 and RT-4 cells than the normal bladder epithelial CP-H068 cells (P < 0.05). NPRL2 silencing resulted in decreased proliferation ability, elevated apoptotic rate, and increased protein levels of JNK, Bax and caspase-9, while decreased level of Bcl-2 in the Biu87 and RT-4 cells (P < 0.05). Conclusion The expression of NPRL2 is related to the clinical stage and grade of bladder cancer. Silencing NPRL2 in bladder cancer cells Biu87/RT-4 can effectively inhibit the proliferation and promote apoptosis of bladder cancer cells.

Keywords

• nprl2
• bladder cancer
• biu87 cells
• rt-4 cells
• apoptosis