Development of Microsatellite Loci in <i>Scrophularia incisa</i> (Scrophulariaceae) and Cross-Amplification in Congeneric Species

Abstract

Premise of the study: To elucidate the population genetics and phylogeography of Scrophularia incisa, microsatellite primers were developed. We also applied these microsatellite markers to its closely related species S. dentata and S. kiriloviana. Methods and Results: Using the compound microsatellite marker technique, 12 microsatellite primers were identified in S. incisa. The number of alleles ranged from 14 to 26 when assessed in 78 individuals from four populations. With high cross-species transferability, these primers also amplified in S. dentata and S. kiriloviana. Conclusions: These results indicate that these microsatellite markers are adequate for detecting and characterizing population genetic structure in the Chinese species of sect. Tomiophyllum at fine and range-wide geographical scales.

Keywords

• genetic diversity
• medicinal herb
• microsatellite
• Qinghai–Tibet Plateau
• Scrophularia dentata
• Scrophularia kiriloviana