Nature Communications (Jul 2021)
The role of membrane destabilisation and protein dynamics in BAM catalysed OMP folding
- Paul White,
- Samuel F. Haysom,
- Matthew G. Iadanza,
- Anna J. Higgins,
- Jonathan M. Machin,
- James M. Whitehouse,
- Jim E. Horne,
- Bob Schiffrin,
- Charlotte Carpenter-Platt,
- Antonio N. Calabrese,
- Kelly M. Storek,
- Steven T. Rutherford,
- David J. Brockwell,
- Neil A. Ranson,
- Sheena E. Radford
Affiliations
- Paul White
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Samuel F. Haysom
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Matthew G. Iadanza
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Anna J. Higgins
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Jonathan M. Machin
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- James M. Whitehouse
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Jim E. Horne
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Bob Schiffrin
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Charlotte Carpenter-Platt
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Antonio N. Calabrese
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Kelly M. Storek
- Department of Infectious Diseases, Genentech Inc.
- Steven T. Rutherford
- Department of Infectious Diseases, Genentech Inc.
- David J. Brockwell
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Neil A. Ranson
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- Sheena E. Radford
- Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds
- DOI
- https://doi.org/10.1038/s41467-021-24432-x
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 13
Abstract
The folding of outer membrane proteins (OMPs) is catalyzed by the βbarrel assembly machinery (BAM). Here, structural and functional analyses of BAM stabilized in distinct conformations elucidate the roles of lateral gate opening and interactions of BAM with the lipid bilayer in OMP assembly.
