Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes

Takaaki Tanaka; Yoshihiko Takai; Akifumi Nagase; Kazuki Teraguchi; Hiromi Minbu; Akihito Ochiai; Isao Kimura; Masayuki Taniguchi

Heliyon (Apr 2019)

Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes

Takaaki Tanaka,
Yoshihiko Takai,
Akifumi Nagase,
Kazuki Teraguchi,
Hiromi Minbu,
Akihito Ochiai,
Isao Kimura,
Masayuki Taniguchi

Affiliations

Takaaki Tanaka: Department of Materials Science and Technology, Niigata University, Niigata 950-2181, Japan; Corresponding author.
Yoshihiko Takai: Department of Materials Science and Technology, Niigata University, Niigata 950-2181, Japan
Akifumi Nagase: Department of Materials Science and Technology, Niigata University, Niigata 950-2181, Japan
Kazuki Teraguchi: Department of Materials Science and Technology, Niigata University, Niigata 950-2181, Japan
Hiromi Minbu: Department of Materials Science and Technology, Niigata University, Niigata 950-2181, Japan
Akihito Ochiai: Department of Materials Science and Technology, Niigata University, Niigata 950-2181, Japan
Isao Kimura: Department of Chemistry and Chemical Engineering, Niigata University, Niigata 950-2181, Japan
Masayuki Taniguchi: Department of Materials Science and Technology, Niigata University, Niigata 950-2181, Japan

Journal volume & issue: Vol. 5, no. 4
p. e01490

Abstract

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Nanoparticle-assembled hydroxyapatite (HA) hollow microspheres have a high surface area and are convenient to handle, owing to their characteristic structure. In this study we characterized the protein adsorption of HA hollow microspheres prepared from CaCl2 and K2HPO4 by a water-in-oil-in-water (W/O/W) emulsion method assisted by two surfactants: Span 80 and Tween 20. The HA hollow microspheres adsorbed bovine serum albumin, bovine γ-globulin, equine skeletal muscle myoglobin, and chicken egg white lysozyme in 10 mM sodium phosphate buffer (pH 6.8) in a Langmuir-type adsorption and desorbed the proteins in 800 mM sodium phosphate buffer (pH 6.8). The maximum adsorbed amounts of the HA hollow microspheres were 7.5–9.0 times higher than those of the microrods with a similar size range. The composite membranes of the HA microspheres and the poly(l-lactic acid) (PLLA) microporous membranes exhibited a high adsorption capacity for γ-globulin.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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