Differential Drought Responses of Soybean Genotypes in Relation to Photosynthesis and Growth-Yield Attributes

Abstract

Water scarcity leads to significant ecological challenges for global farming production. Sustainable agriculture depends on developing strategies to overcome the impacts of drought on important crops, including soybean. In this present study, seven promising soybean genotypes were evaluated for their drought tolerance potential by exposing them to water deficit conditions. The control group was maintained at 100% field capacity (FC), while the drought-treated group was maintained at 50% FC on a volume/weight basis. This treatment was applied at the second trifoliate leaf stage and continued until maturity. Our results demonstrated that water shortage exerted negative impacts on soybean phenotypic traits, physiological and biochemical mechanisms, and yield output in comparison with normal conditions. Our results showed that genotype G00001 exhibited the highest leaf area plant−1 (483.70 cm2), photosynthetic attributes like stomatal conductance (gs) (0.15 mol H2O m−2 s−1) and photosynthetic rate (Pn) (13.73 μmol CO2 m−2 s−1), and xylem exudation rate (0.25 g h−1) under drought conditions. The G00001 genotype showed greater leaf greenness by preserving photosynthetic pigments (total chlorophylls (Chls) and carotenoids; 4.23 and 7.34 mg g−1 FW, respectively) in response to drought conditions. Soybean plants accumulated high levels of stress indicators like proline and malondialdehyde when subjected to drought stress. However, genotype G00001 displayed lower levels of proline (4.49 μg g−1 FW) and malondialdehyde (3.70 μmol g−1 FW), indicating that this genotype suffered from less oxidative stress induced by drought stress compared to the other investigated soybean genotypes. Eventually, the G00001 genotype had a greater yield in terms of seeds pod−1 (SP) (1.90) and 100-seed weight (HSW) (14.60 g) under drought conditions. On the other hand, BD2333 exhibited the largest decrease in plant height (37.10%), pod number plant−1 (85.90%), SP (56.20%), HSW (54.20%), gs (90.50%), Pn (71.00%), transpiration rate (59.40%), relative water content (34.40%), Chl a (79.50%), total Chls (72.70%), and carotenoids (56.70%), along with the maximum increase in water saturation deficit (290.40%) and malondialdehyde content (280.30%) under drought compared to control conditions, indicating its higher sensitivity to drought stress. Our findings suggest that G00001 is a promising candidate to consider for field trials and further evaluation of its molecular signature may help breeding other elite cultivars to develop drought-tolerant, high-yielding soybean varieties.

Keywords

• drought
• soybean genotypes
• gas exchange features
• proline
• stress tolerance