OncoTargets and Therapy (Apr 2019)
The proliferation of cervical cancer is promoted by miRNA-125b through the regulation of the HMGA1
Abstract
Bingmei Sun, Ying Zhang, Lianxiang Zhou, Linin Yin, Fei Li, Chao Li, Jiayu Xia Department of Gynaecology and Obstetrics, Linyi Central Hospital, Linyi 276400, Shandong Province, China Background: It has been reported that miRNA-125b is associated with carcinogenesis and development of several different kinds of cancers. Nevertheless, there is no clarity regarding the significance and mechanism of action of miR-125b in clinical practice for cervical cancer (CC). Materials and methods: In the current investigation, the expression of miR-125b in cervical clinical specimens and CC cell lines was analyzed via real-time quantitative PCR, and the relationship of miR-125b with the chromatin-associated protein high mobility group A (HMGA1) expression and clinicopathological parameters of CC patients was explored. Results: The results indicated that miR-125b expression was remarkably upregulated in CC cell lines as well as in the tissues of humans. miR-125b overexpression was significantly related to a decrease in HMGA1 expression, progression-free survival, overall survival, and prognosis as well. Besides, knockdown of miR-125b inhibited proliferation and colony formation in SW756 and C4-1 cells, where the 3'-UTR of HMGA1 mRNA was directly targeted. Moreover, PI3K/Akt pathway was regulated by miR-125b through suppression of HMGA1. Conclusion: These findings illustrated that a new regulatory role of HMGA1 is involved in the progression of CC. Our data demonstrated that miR-125b could play a critical role in the carcinogenesis and progression of CC, revealing that miR-125b might serve as a potential new target for treating CC. Keywords: cervical cancer, CC, miR-125b, high mobility group A, HMGA1, progression-free survival, PFS, overall survival, OS, prognosis
