Genome-wide identification of flowering Chinese cabbage BPC family genes and BcBPC9 functional analysis in Cd stress tolerance

Shuaiwei Zhang; Jinmiao Wang; Yunqiang Feng; Yanxu Xue; Yudan Wang; Mingfeng Zhao; Muxi Chen; Changming Chen; Wei Su; Riyuan Chen; Ali Anwar; Shiwei Song

Plant Stress (Dec 2023)

Genome-wide identification of flowering Chinese cabbage BPC family genes and BcBPC9 functional analysis in Cd stress tolerance

Shuaiwei Zhang,
Jinmiao Wang,
Yunqiang Feng,
Yanxu Xue,
Yudan Wang,
Mingfeng Zhao,
Muxi Chen,
Changming Chen,
Wei Su,
Riyuan Chen,
Ali Anwar,
Shiwei Song

Affiliations

Shuaiwei Zhang: College of Horticulture, South China Agricultural University, Guangzhou, China
Jinmiao Wang: College of Horticulture, South China Agricultural University, Guangzhou, China
Yunqiang Feng: College of Horticulture, South China Agricultural University, Guangzhou, China
Yanxu Xue: College of Horticulture, South China Agricultural University, Guangzhou, China
Yudan Wang: College of Horticulture, South China Agricultural University, Guangzhou, China
Mingfeng Zhao: College of Horticulture, South China Agricultural University, Guangzhou, China
Muxi Chen: Guangdong Helinong Biological Seeds, CO.LTD, Shantou, Guangdong 515800, P.R. China
Changming Chen: College of Horticulture, South China Agricultural University, Guangzhou, China
Wei Su: College of Horticulture, South China Agricultural University, Guangzhou, China
Riyuan Chen: College of Horticulture, South China Agricultural University, Guangzhou, China
Ali Anwar: College of Horticulture, South China Agricultural University, Guangzhou, China; Corresponding authors.
Shiwei Song: College of Horticulture, South China Agricultural University, Guangzhou, China; Corresponding authors.

Journal volume & issue: Vol. 10
p. 100220

Abstract

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Flowering Chinese cabbage is an important vegetable crop widely cultivated in southern China. However, flowering Chinese cabbage plants are susceptible to diverse environmental influences that mainly affect crop quality and production. The BASIC PENTACYSTEINE (BPC) transcription factor is an essential regulator of plant development and abiotic stress responses. However, the function and molecular mechanism of the BPC family genes in flowering Chinese cabbage remain unclear. This study aimed to investigate the molecular mechanisms of the BcBPC gene in abiotic stress tolerance. In flowering Chinese cabbage, 12 BcBPC family genes have been identified and found to be unequally distributed on eight chromosomes. Comprehensive analyses of BcBPC gene structure, motif analysis, cis-regulatory elements, and subcellular localization were performed. BcBPC genes were classified into three groups based on their sequence composition, phylogenetic relationships, and conserved domains, which were highly linked to those of other species. BcBPC family gene promoter consists 69.92% stress-responsive, 32.38% hormone-responsive, and 5.30%, related to growth- and biological process-responsive cis-regulatory elements. Subsequent qRT-PCR results showed that BcBPC genes were highly upregulated under abiotic stress, especially under NaCl and Cd stress. The overexpression of BcBPC9 increases Cd stress tolerance in yeast. BcBPC9 is located in the nucleus, but moves to the cell wall when exposed to Cd stress. BcBPC9 transient overexpression tobacco were improved growth and upregulated the antioxidant enzymes genes expression when exposed to Cd stress. These findings facilitate further investigation of the functional and molecular characteristics of BcBPC9 in response to abiotic stress. The outcomes of this study provide a crucial foundation for future research on improving plant growth and protecting vegetable production.

Published in Plant Stress

ISSN: 2667-064X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Botany: Plant ecology
Website: https://www.journals.elsevier.com/plant-stress/

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