Identification and Biochemical Characterization of some Strains of Clavibacter michiganensis subsp. Michiganensis, in Romania

Abstract

Clavibacter michiganensis subsp. michiganensis is responsible for the ʻbacterial canker of tomato’. The study aimed to identify and characterize biochemically some strains of the bacteria. Five strains were used for biochemical identification and characterization and two reference strains were used as positive controls. The biochemical characterization was performed with Biolog Microbial Identification System. All the strains used many carbon sources and were positive for growth in the presence of: 1%NaCl, Nalidixic Acid, Aztreonam, Potassium Tellurite and pH 6, but negative for growth and sensitivite to: Troleandomycin, Lincomycin, Vancomycin, Fusidic Acid, Eimfamycin SV, Guanidine HCl, D-Serine, Minocycline and Niaproof 4. The differences between the Biolog system database and the studied strains did not change their classification at the level of genus and species, but modifed or did not allow the classification at the subspecies level. Furthermore, the analyzed strains had metabolic diversity.

Keywords

• biolog system
• spectrophotometry
• optic.