Nature Communications (Sep 2022)

Metabolic pathway assembly using docking domains from type I cis-AT polyketide synthases

  • Xixi Sun,
  • Yujie Yuan,
  • Qitong Chen,
  • Shiqi Nie,
  • Jiaxuan Guo,
  • Zutian Ou,
  • Min Huang,
  • Zixin Deng,
  • Tiangang Liu,
  • Tian Ma

DOI
https://doi.org/10.1038/s41467-022-33272-2
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 12

Abstract

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Assembly artificial pathway in design connecting media can increase biosynthetic efficiency, but the choice of connecting media is limited. Here, the authors develop a new protein assembly strategy using a pool of docking peptides from polyketide synthase and show its application in astaxanthin biosynthesis in E. coli.