Nature Communications (Aug 2021)

CTCF is a barrier for 2C-like reprogramming

  • Teresa Olbrich,
  • Maria Vega-Sendino,
  • Desiree Tillo,
  • Wei Wu,
  • Nicholas Zolnerowich,
  • Raphael Pavani,
  • Andy D. Tran,
  • Catherine N. Domingo,
  • Mariajose Franco,
  • Marta Markiewicz-Potoczny,
  • Gianluca Pegoraro,
  • Peter C. FitzGerald,
  • Michael J. Kruhlak,
  • Eros Lazzerini-Denchi,
  • Elphege P. Nora,
  • André Nussenzweig,
  • Sergio Ruiz

DOI
https://doi.org/10.1038/s41467-021-25072-x
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 12

Abstract

Read online

Embryos at the 2-cell (2C) stage are totipotent, and overexpression of Dux transcription factor convert embryonic stem cells (ESCs) to a 2C-like state. Here the authors show that DUX-mediated 2C-like reprogramming is associated with DNA damage at CTCF sites and CTCF depletion promotes 2Clike conversion.