Acta Cirúrgica Brasileira (Mar 2015)

Use of low-power laser to assist the healing of traumatic wounds in rats

  • Fernanda Camila Ferreira da Silva Calisto,
  • Sérgio Luís da Silva Calisto,
  • Amanda Pires de Souza,
  • Cristiane Miranda França,
  • Ana Paula de Lima Ferreira,
  • Márcia Bento Moreira

DOI
https://doi.org/10.1590/S0102-865020150030000007
Journal volume & issue
Vol. 30, no. 3
pp. 204 – 208

Abstract

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PURPOSE: To investigate the morphological aspects of the healing of traumatic wounds in rats using low-power laser.METHODS:Twenty four non isogenic, young adult male Wistar rats (Rattus norvegicus) weighing between 200 and 300g was used. The animals were randomly distributed into two groups: Control (GC) and Laser (GL), with 12 animals each. After shaving, anesthesia was performed in the dorsal region and then a surgical procedure using a scapel was carried out to make the traumatic wound. GL received five sessions of laser therapy in consecutive days using the following laser parameters: wavelength 660 nm, power 100 mW, dose 10 J/cm2. The wounds were evaluated through measurement of the area and depth of the wound (MW) and histological analysis (HA).RESULTS:When comparing the GC with the GL in MW there was a difference in area (p<0.001) and depth (p=0.003) measurement of the wounds in GL. The laser group presented more epithelization than GC (p=0.03). The other histological parameters were similar.CONCLUSION:The healing of wounds in rats was improved with the use of the laser.

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