Frontiers in Molecular Neuroscience (Jan 2019)
Circular RNA Expression Alteration and Bioinformatics Analysis in Rats After Traumatic Spinal Cord Injury
- Chuan Qin,
- Chuan Qin,
- Chuan Qin,
- Chuan Qin,
- Chuan Qin,
- Chang-Bin Liu,
- Chang-Bin Liu,
- Chang-Bin Liu,
- Chang-Bin Liu,
- Chang-Bin Liu,
- De-Gang Yang,
- De-Gang Yang,
- De-Gang Yang,
- De-Gang Yang,
- De-Gang Yang,
- Feng Gao,
- Feng Gao,
- Feng Gao,
- Feng Gao,
- Feng Gao,
- Xin Zhang,
- Xin Zhang,
- Xin Zhang,
- Xin Zhang,
- Xin Zhang,
- Chao Zhang,
- Chao Zhang,
- Chao Zhang,
- Chao Zhang,
- Chao Zhang,
- Liang-Jie Du,
- Liang-Jie Du,
- Liang-Jie Du,
- Liang-Jie Du,
- Liang-Jie Du,
- Ming-Liang Yang,
- Ming-Liang Yang,
- Ming-Liang Yang,
- Ming-Liang Yang,
- Ming-Liang Yang,
- Jian-Jun Li,
- Jian-Jun Li,
- Jian-Jun Li,
- Jian-Jun Li,
- Jian-Jun Li
Affiliations
- Chuan Qin
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Chuan Qin
- China Rehabilitation Science Institute, Beijing, China
- Chuan Qin
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Chuan Qin
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Chuan Qin
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Chang-Bin Liu
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Chang-Bin Liu
- China Rehabilitation Science Institute, Beijing, China
- Chang-Bin Liu
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Chang-Bin Liu
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Chang-Bin Liu
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- De-Gang Yang
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- De-Gang Yang
- China Rehabilitation Science Institute, Beijing, China
- De-Gang Yang
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- De-Gang Yang
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- De-Gang Yang
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Feng Gao
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Feng Gao
- China Rehabilitation Science Institute, Beijing, China
- Feng Gao
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Feng Gao
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Feng Gao
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Xin Zhang
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Xin Zhang
- China Rehabilitation Science Institute, Beijing, China
- Xin Zhang
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Xin Zhang
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Xin Zhang
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Chao Zhang
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Chao Zhang
- China Rehabilitation Science Institute, Beijing, China
- Chao Zhang
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Chao Zhang
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Chao Zhang
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Liang-Jie Du
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Liang-Jie Du
- China Rehabilitation Science Institute, Beijing, China
- Liang-Jie Du
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Liang-Jie Du
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Liang-Jie Du
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Ming-Liang Yang
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Ming-Liang Yang
- China Rehabilitation Science Institute, Beijing, China
- Ming-Liang Yang
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Ming-Liang Yang
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Ming-Liang Yang
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Jian-Jun Li
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Jian-Jun Li
- China Rehabilitation Science Institute, Beijing, China
- Jian-Jun Li
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Jian-Jun Li
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Jian-Jun Li
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- DOI
- https://doi.org/10.3389/fnmol.2018.00497
- Journal volume & issue
-
Vol. 11
Abstract
Spinal cord injury (SCI) is mostly caused by trauma. As primary mechanical injury is unavoidable in SCI, a focus on the pathophysiology and underlying molecular mechanisms of SCI-induced secondary injury is necessary to develop promising treatments for SCI patients. Circular RNAs (circRNAs) are associated with various diseases. Nevertheless, studies to date have not yet determined the functional roles of circRNAs in traumatic SCI. We examined circRNA expression profiles in the contused spinal cords of rats using microarray and quantitative reverse transcription-PCR (qRT-PCR) then predict their potential roles in post-SCI pathophysiology with bioinformatics. We found a total of 1676 differentially expressed circRNAs (fold change ≥ 2.0; P < 0.05) in spinal cord 3 days after contusion using circRNA microarray; 1261 circRNAs were significantly downregulated, whereas the remaining 415 were significantly upregulated. Then, five selected circRNAs, namely, rno_circRNA_005342, rno_circRNA_015513, rno_circRNA_002948, rno_circRNA_006096, and rno_circRNA_013017 were all significantly downregulated in the SCI group after verification by qRT-PCR, demonstrating a similar expression pattern in both microarray and PCR data. The next section of the study was concerned with the prediction of circRNA/miRNA/mRNA interactions using bioinformatics analysis. In the final part of the study, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses indicated carbohydrate metabolic process was one of the most significant enrichments and meaningful terms after GO analysis, and the top two signaling pathways affected by the circRNAs-miRNAs axes were the AMP-activated protein kinase signaling pathway and the peroxisome related pathway. In summary, this study showed an altered circRNA expression pattern that may be involved in physiological and pathological processes in rats after traumatic SCI, providing deep insights into numerous possibilities for SCI treatment targets by regulating circRNAs.
Keywords