Effect of Selenium Nanoparticles and Mannan Oligosaccharide Supplementation on Growth Performance, Stress Indicators, and Intestinal Microarchitecture of Broilers Reared under High Stocking Density
Hafiz Faseeh ur Rehman,
Hafsa Zaneb,
Saima Masood,
Muhammad Shahbaz Yousaf,
Khizar Hayat,
Khalid Abdul Majeed,
Muhammad Zeeshan,
Saima Ashraf,
Imad Khan,
Adnan Khan,
Habib Rehman
Affiliations
Hafiz Faseeh ur Rehman
Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
Hafsa Zaneb
Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
Saima Masood
Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
Muhammad Shahbaz Yousaf
Department of Physiology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
Khizar Hayat
Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
Khalid Abdul Majeed
Department of Physiology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
Muhammad Zeeshan
Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
Saima Ashraf
Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
Imad Khan
College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan 23200, Pakistan
Adnan Khan
Institute of Chemical Sciences, University of Peshawar, Peshawar 25000, Pakistan
Habib Rehman
Department of Physiology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
The current study investigated the potential of selenium nanoparticles (SeNPs) and mannan-oligosaccharide (MOS) supplementation in ameliorating high stocking density (HSD) stress in broilers. A total of 392 day-old male chicks were divided into seven groups with eight replicates (n = 7): NSD [basal diet (BD) + normal stocking density: 10 bird/m2], HSD [BD + high stocking density: 16 bird/m2], Se–HSD [BD + Selenium (Se) 0.15 mg/kg], MOS–HSD (BD + MOS 5 gm/kg), Se–MOS–HSD (BD + Se 0.15 mg/kg and MOS 5 gm/kg), SeNPs–HSD (BD + SeNPs 0.15 mg/kg) and SeNPs–MOS–HSD (BD + SeNPs 0.15 mg/kg and MOS-5 gm/kg). HSD stress decreased (p p p p p p p p p p p < 0.05) serum cholesterol and ileal IEL density in HSD-stressed broilers. Our findings signify that HSD is stressful for broilers particularly during the finishing phase. Supplementation with the SeNPs–MOS combination mitigated HSD stress by partially improving the gut microarchitecture, gut barrier function and performance indicators.
