EFFECTS OF LOW-INTENSITY MICROWAVE IRRADIATION UPON INTRACELLULAR SIGNALING OF MONONUCLEAR CELLS IN PNEUMONIA PATIENTS

Abstract

Abstract. Some molecular biomarkers of peripheral blood mononuclear cells (PBMC) were studied in patients 18 to 30 years old with community-acquired pneumonia (n = 30) and healthy persons (n = 15), along with testing appropriate biological effects of low-intensity microwave irradiation (1000 MHz), at a dose rate of 100 pW/cm2. Concentrations of cytokines and some molecules providing intracellular signal transduction in PBMC were measured by ELISA tests. Biological action of microwave radiation was studied after 1-hour irradiation of whole blood, followed by incubation for 24 hours. A single round of irradiation was shown to cause an increase in NFκB content by 12.5% (р = 0.001); IκB by 21.1% (р = 0.0007); phosphorylated JNK1/2, by 18.2% (р = 0.052); p21 protein amounts, by 56.2% (р = 0.031); IL-2, by 8.5% (р = 0.08); IL-4, by 17.6% (р = 0.031). Antioxidant potential of PBMC supernatewas augmented by 65.2% (р < 0,001). Hence, a single 1000-MHz microwave irradiation modulates activity of intracellular events in PBMCs from pneumonia patients.

Keywords

• microwave radiation
• mononuclear cells
• cytokines
• transcription factors
• pneumonia