Ultrasound–Microwave Combined Extraction of Novel Polysaccharide Fractions from <i>Lycium barbarum</i> Leaves and Their In Vitro Hypoglycemic and Antioxidant Activities

Na Quan; Yi-Dan Wang; Guo-Rong Li; Zi-Qi Liu; Jing Feng; Chun-Lei Qiao; Hua-Feng Zhang

doi:10.3390/molecules28093880

Molecules (May 2023)

Ultrasound–Microwave Combined Extraction of Novel Polysaccharide Fractions from <i>Lycium barbarum</i> Leaves and Their In Vitro Hypoglycemic and Antioxidant Activities

Na Quan,
Yi-Dan Wang,
Guo-Rong Li,
Zi-Qi Liu,
Jing Feng,
Chun-Lei Qiao,
Hua-Feng Zhang

Affiliations

Na Quan: International Joint Research Center of Shaanxi Province for Food and Health Sciences, Provincial Research Station of Se-Enriched Foods in Hanyin County of Shaanxi Province, College of Food Engineering and Nutritional Science, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Shaanxi Normal University, Xi’an 710119, China
Yi-Dan Wang: International Joint Research Center of Shaanxi Province for Food and Health Sciences, Provincial Research Station of Se-Enriched Foods in Hanyin County of Shaanxi Province, College of Food Engineering and Nutritional Science, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Shaanxi Normal University, Xi’an 710119, China
Guo-Rong Li: Yinchuan Market Supervision Administration, Yinchuan 750001, China
Zi-Qi Liu: International Joint Research Center of Shaanxi Province for Food and Health Sciences, Provincial Research Station of Se-Enriched Foods in Hanyin County of Shaanxi Province, College of Food Engineering and Nutritional Science, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Shaanxi Normal University, Xi’an 710119, China
Jing Feng: Agrarian and Technological Institute, Peoples’ Friendship University of Russia, Moscow 119991, Russia
Chun-Lei Qiao: International Joint Research Center of Shaanxi Province for Food and Health Sciences, Provincial Research Station of Se-Enriched Foods in Hanyin County of Shaanxi Province, College of Food Engineering and Nutritional Science, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Shaanxi Normal University, Xi’an 710119, China
Hua-Feng Zhang: International Joint Research Center of Shaanxi Province for Food and Health Sciences, Provincial Research Station of Se-Enriched Foods in Hanyin County of Shaanxi Province, College of Food Engineering and Nutritional Science, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Shaanxi Normal University, Xi’an 710119, China

DOI: https://doi.org/10.3390/molecules28093880
Journal volume & issue: Vol. 28, no. 9
p. 3880

Abstract

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Ultrasound–microwave combined extraction (UMCE), gradient ethanol precipitation, chemical characterization, and antioxidant and hypoglycemic activities of Lycium barbarum leaf polysaccharides (LLP) were systematically studied. The optimal conditions for UMCE of LLP achieved by response surface method (RSM) were as follows: microwave time of 16 min, ultrasonic time of 20 min, particle size of 100 mesh, and ratio of liquid to solid of 55:1. Three novel polysaccharide fractions (LLP30, LLP50, LLP70) with different molecular weights were obtained by gradient ethanol precipitation. Polysaccharide samples exhibited scavenging capacities against ABTS and DPPH radicals and inhibitory activities against α-glucosidase and α-amylase. Among the three fractions, LLP30 possessed relatively high antioxidant and hypoglycemic activities in vitro, which showed a potential for becoming a nutraceutical or a phytopharmaceutical for prevention and treatment of hyperglycemia or diabetes.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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