The correlation between individual biochemical parameters of functional state of the liver in experimental obstructive jaundice and course administration of L-lysine escinate and glutargine

Medičnì Perspektivi. 2016;21(4):8-15

 

Journal Homepage

Journal Title: Medičnì Perspektivi

ISSN: 2307-0404 (Print)

Publisher: SE "Dnipropetrovsk medical academy of Health Ministry of Ukraine"

Society/Institution: SE "Dnipropetrovsk medical academy of Health Ministry of Ukraine"

LCC Subject Category: Medicine: Medicine (General)

Country of publisher: Ukraine

Language of fulltext: Russian, Ukrainian

Full-text formats available: PDF

 

AUTHORS

Desjateryk V.I. (SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine» )
Mamchur D.V. (SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine» )
Kotov O.V. (SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine» )
Dronov S.M. (SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine» )
Zhyliuk V.I. (SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine» )

EDITORIAL INFORMATION

Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 4 weeks

 

Abstract | Full Text

The aim of this study was to determine the correlations between markers of oxidant-antioxidant stress in the blood of experimental animals, as well as the content of bilirubin and activity of enzymes in experimental obstructive jaundice. Studies were conducted on 84 adult male Wistar rats (180-230 g). Animals were divided into 4 groups: intact (n = 12), control (n = 24), L-lysine escinate (0,5 mg/kg, n = 24) and glutargin (40 mg/kg, n = 24). The isolation and ligation of the common bile duct below confluence was carried out under general anesthesia. The test preparations were administered 1 time per day, intraperitoneally. Evaluation of the treatment efficacy was carried out on 4th and 10th day of the study. The content of malonic dialdehyde (MDA), aldehyde- (APH) and ketone-phenylhydrazones (CPH), bilirubin and the activity of superoxide dismutase (SOD), catalase, aspartate aminotransferase (ALT) and alanine aminotransferase (AST) in the blood were determined using conventional techniques. The correlation links between common markers (bilirubin, AST, ALT) and indicators characterizing oxidant-antioxidant stress (MDA, APH, CPH, SOD, catalase) were determined in the study. High rate of correlation between them was established. The results allow to evaluate indirectly the intensity of oxidative metabolism processes in the liver, which can be used in clinical practice to assess the severity degree of the patients with progressive obstructive jaundice.