Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution

Yibin Liu; Zhiyuan Hu; Jingfei Cheng; Paulina Siejka-Zielińska; Jinfeng Chen; Masato Inoue; Ahmed Ashour Ahmed; Chun-Xiao Song

doi:10.1038/s41467-021-20920-2

Nature Communications (Jan 2021)

Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution

Yibin Liu,
Zhiyuan Hu,
Jingfei Cheng,
Paulina Siejka-Zielińska,
Jinfeng Chen,
Masato Inoue,
Ahmed Ashour Ahmed,
Chun-Xiao Song

Affiliations

Yibin Liu: Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of Oxford
Zhiyuan Hu: Ovarian Cancer Cell Laboratory, MRC Weatherall Institute of Molecular Medicine, University of Oxford
Jingfei Cheng: Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of Oxford
Paulina Siejka-Zielińska: Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of Oxford
Jinfeng Chen: Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of Oxford
Masato Inoue: Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of Oxford
Ahmed Ashour Ahmed: Ovarian Cancer Cell Laboratory, MRC Weatherall Institute of Molecular Medicine, University of Oxford
Chun-Xiao Song: Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of Oxford

DOI: https://doi.org/10.1038/s41467-021-20920-2
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 8

Abstract

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Specific and quantitative sequencing of cytosine modifications is challenging at base-resolution. Here the authors present TAPSβ and CAPS for subtraction-free whole genome sequencing of 5mC and 5hmC.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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