Optimization PHAs production from dairy industry wastewater (cheese whey) by Azohydromonas lata DSMZ 1123

Abstract

In the present research, whey was used as useful substrate which retained from permeates of dairy industry. The obtained whey was hydrolyzed to cleave its main carbon source, lactose to glucose and galactose.The hydrolyzed products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by Azohydromonas lata DSMZ 1123. The biosynthesis of PHA copolyesters containing 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units from hydrolyzed whey permeate and valerate were investigated. The application of hydrolyzed whey permeate turned out to be advantageous compared to utilization of pure sugars. Therefore, fermentation under controlled conditions (agitation rate of 250 rpm, 18 h incubated inoculum, 72 h fermentation time and temperature set at 30°C) was performed. As a result, maximum amount biopolymer obtained was1.66 g/L. The biopolymer consisted 1.21 and 0.45 g/L of P(3HB) and P(3HV), respectively.

Keywords

• Azohydromonas lata
• Cheese whey
• Lactose
• 3-hydroxybutyrate (3HB)
• 3-hydroxyvalerate (3HV)
• Polyhydroxyalkanoate