pH and ionic strength triggered destabilization of biocompatible stable water-in-oil-in-water (W/O/W) emulsions
Marine Protat,
Noémie Bodin-Thomazo,
Frédéric Gobeaux,
Florent Malloggi,
Jean Daillant,
Nadège Pantoustier,
Patrick Perrin,
Patrick Guenoun
Affiliations
Marine Protat
Sciences et Ingénierie de la Matière Molle, PSL Research University, ESPCI Paris, CNRS, 10 rue Vauquelin, 75231, Paris cedex 05, France; Université Paris-Saclay, CEA, CNRS, NIMBE, LIONS, 91191, Gif-sur-Yvette, France
Noémie Bodin-Thomazo
Sciences et Ingénierie de la Matière Molle, PSL Research University, ESPCI Paris, CNRS, 10 rue Vauquelin, 75231, Paris cedex 05, France; Université Paris-Saclay, CEA, CNRS, NIMBE, LIONS, 91191, Gif-sur-Yvette, France
Frédéric Gobeaux
Université Paris-Saclay, CEA, CNRS, NIMBE, LIONS, 91191, Gif-sur-Yvette, France
Florent Malloggi
Université Paris-Saclay, CEA, CNRS, NIMBE, LIONS, 91191, Gif-sur-Yvette, France
Jean Daillant
Synchrotron Soleil, L'Orme des Merisiers, Saint-Aubin – BP 48, 91192, Gif-sur-Yvette Cedex, France
Nadège Pantoustier
Sciences et Ingénierie de la Matière Molle, PSL Research University, ESPCI Paris, CNRS, 10 rue Vauquelin, 75231, Paris cedex 05, France; Sciences et Ingénierie de la Matière Molle, Sorbonne Universités, Université Pierre et Marie Curie, Paris, 06, 10 rue Vauquelin, France
Patrick Perrin
Sciences et Ingénierie de la Matière Molle, PSL Research University, ESPCI Paris, CNRS, 10 rue Vauquelin, 75231, Paris cedex 05, France; Sciences et Ingénierie de la Matière Molle, Sorbonne Universités, Université Pierre et Marie Curie, Paris, 06, 10 rue Vauquelin, France; Corresponding author. Sciences et Ingénierie de la Matière Molle, PSL Research University, ESPCI Paris, CNRS, 10 rue Vauquelin, 75231, Paris cedex 05, France.
The design of biocompatible multiple emulsions is an important challenge in the field of controlled delivery systems for protecting and delivering compounds encapsulated and protected in the innermost phase. In this paper, we use biocompatible water – Miglyol®812 water-in-oil-in-water (W/O/W) emulsions stabilized by a stimuli-responsive diblock copolymer consisting of poly(dimethylsiloxane) (PDMS) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) to design an easy-to-process new delivery W/O/W system. Such emulsions are formed in a single emulsification step. They present a high encapsulation yield and are shown to be stable over months. As such, the encapsulation of a hydrophilic dye (Alexa fluor) in the innermost water phase is successfully demonstrated over months. These emulsions are stimulable either by a shift in pH level or in ionic strength. The former destabilizes the multiple emulsion and leads to a simple one while the latter partly maintains the multiple character. Eventually both stimulations are effective in the dye release and molecular mechanisms are proposed for explaining the observed two-stage kinetics of release.
