Genetic Variations <i>miR-10a</i>A>T, <i>miR-30c</i>A>G, <i>miR-181a</i>T>C, and <i>miR-499b</i>A>G and the Risk of Recurrent Pregnancy Loss in Korean Women
Hui-Jeong An,
Sung-Hwan Cho,
Han-Sung Park,
Ji-Hyang Kim,
Young-Ran Kim,
Woo-Sik Lee,
Jung-Ryeol Lee,
Seong-Soo Joo,
Eun-Hee Ahn,
Nam-Keun Kim
Affiliations
Hui-Jeong An
Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea
Sung-Hwan Cho
Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea
Han-Sung Park
Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea
Ji-Hyang Kim
Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam 13488, Korea
Young-Ran Kim
Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam 13488, Korea
Woo-Sik Lee
Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul 06135, Korea
Jung-Ryeol Lee
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam 13620, Korea
Seong-Soo Joo
College of Life Science, Gangneung-Wonju National University, Gangneung 25457, Korea
Eun-Hee Ahn
Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam 13488, Korea
Nam-Keun Kim
Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea
This study investigated the genetic association between recurrent pregnancy loss (RPL) and microRNA (miRNA) polymorphisms in miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G in Korean women. Blood samples were collected from 381 RPL patients and 281 control participants, and genotyping of miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G was carried out by TaqMan miRNA RT-Real Time polymerase chain reaction (PCR). Four polymorphisms were identified, including miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G. MiR-10a dominant model (AA vs. AT + TT) and miR-499bGG genotypes were associated with increased RPL risk (adjusted odds ratio [AOR] = 1.520, 95% confidence interval [CI] = 1.038–2.227, p = 0.032; AOR = 2.956, 95% CI = 1.168–7.482, p = 0.022, respectively). Additionally, both miR-499 dominant (AA vs. AG + GG) and recessive (AA + AG vs. GG) models were significantly associated with increased RPL risk (AOR = 1.465, 95% CI = 1.062–2.020, p = 0.020; AOR = 2.677, 95% CI = 1.066–6.725, p = 0.036, respectively). We further propose that miR-10aA>T, miR-30cA>G, and miR-499bA>G polymorphisms effects could contribute to RPL and should be considered during RPL patient evaluation.