Biomedicines (Sep 2022)

Genetic Variations <i>miR-10a</i>A>T, <i>miR-30c</i>A>G, <i>miR-181a</i>T>C, and <i>miR-499b</i>A>G and the Risk of Recurrent Pregnancy Loss in Korean Women

  • Hui-Jeong An,
  • Sung-Hwan Cho,
  • Han-Sung Park,
  • Ji-Hyang Kim,
  • Young-Ran Kim,
  • Woo-Sik Lee,
  • Jung-Ryeol Lee,
  • Seong-Soo Joo,
  • Eun-Hee Ahn,
  • Nam-Keun Kim

DOI
https://doi.org/10.3390/biomedicines10102395
Journal volume & issue
Vol. 10, no. 10
p. 2395

Abstract

Read online

This study investigated the genetic association between recurrent pregnancy loss (RPL) and microRNA (miRNA) polymorphisms in miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G in Korean women. Blood samples were collected from 381 RPL patients and 281 control participants, and genotyping of miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G was carried out by TaqMan miRNA RT-Real Time polymerase chain reaction (PCR). Four polymorphisms were identified, including miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G. MiR-10a dominant model (AA vs. AT + TT) and miR-499bGG genotypes were associated with increased RPL risk (adjusted odds ratio [AOR] = 1.520, 95% confidence interval [CI] = 1.038–2.227, p = 0.032; AOR = 2.956, 95% CI = 1.168–7.482, p = 0.022, respectively). Additionally, both miR-499 dominant (AA vs. AG + GG) and recessive (AA + AG vs. GG) models were significantly associated with increased RPL risk (AOR = 1.465, 95% CI = 1.062–2.020, p = 0.020; AOR = 2.677, 95% CI = 1.066–6.725, p = 0.036, respectively). We further propose that miR-10aA>T, miR-30cA>G, and miR-499bA>G polymorphisms effects could contribute to RPL and should be considered during RPL patient evaluation.

Keywords