Developmental toxicity of bromoacetamide via the thyroid hormone receptors-mediated disruption of thyroid hormone homeostasis in zebrafish embryos

Wei Wang; Qiyao Ma; Xinliang Ding; Yihua Xu; Mengting He; Jie Xu; Jianjun Liu; Cheng Ji; Jie Zhang

Ecotoxicology and Environmental Safety (Mar 2022)

Developmental toxicity of bromoacetamide via the thyroid hormone receptors-mediated disruption of thyroid hormone homeostasis in zebrafish embryos

Wei Wang,
Qiyao Ma,
Xinliang Ding,
Yihua Xu,
Mengting He,
Jie Xu,
Jianjun Liu,
Cheng Ji,
Jie Zhang

Affiliations

Wei Wang: Medical College of Soochow University, Suzhou, China; Department of Toxicology, School of Public Health, Medical College of Soochow University, Suzhou, 215123 Jiangsu, China
Qiyao Ma: Medical College of Soochow University, Suzhou, China; Department of Toxicology, School of Public Health, Medical College of Soochow University, Suzhou, 215123 Jiangsu, China
Xinliang Ding: Department of Public Health, Wuxi Center for Disease Control and Prevention, Wuxi, 214023 Jiangsu, China
Yihua Xu: Medical College of Soochow University, Suzhou, China
Mengting He: Medical College of Soochow University, Suzhou, China; Department of Toxicology, School of Public Health, Medical College of Soochow University, Suzhou, 215123 Jiangsu, China
Jie Xu: Medical College of Soochow University, Suzhou, China; Department of Toxicology, School of Public Health, Medical College of Soochow University, Suzhou, 215123 Jiangsu, China
Jianjun Liu: Medical College of Soochow University, Suzhou, China; Department of Toxicology, School of Public Health, Medical College of Soochow University, Suzhou, 215123 Jiangsu, China
Cheng Ji: Medical College of Soochow University, Suzhou, China; Correspondence to: School of Biology and Basic Medical Sciences, Medical College of Soochow University, 199 Renai Rd, Suzhou, Jiangsu 215123, China.
Jie Zhang: Medical College of Soochow University, Suzhou, China; Department of Toxicology, School of Public Health, Medical College of Soochow University, Suzhou, 215123 Jiangsu, China; Correspondence to: School of Public Health, Medical College of Soochow University, 199 Renai Rd, Suzhou, Jiangsu 215123 China.

Journal volume & issue: Vol. 233
p. 113334

Abstract

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Bromoacetamide (BAcAm) is a nitrogenous disinfection by-product. We previously found that BAcAm induced developmental toxicity in zebrafish embryos, but the underlying mechanisms remain to be elucidated. Since thyroid hormones (THs) homeostasis is crucial to development, we hypothesized that disruption of THs homeostasis may play a role in the developmental toxicity of BAcAm. In this study, we found BAcAm exposure significantly increased mortality and malformation rate, decreased hatching rate and body length, inhibited the locomotor capacity in zebrafish embryos. BAcAm elevated TSH, T3 and T4 levels, down-regulated T3/T4 ratios, and up-regulated mRNA expression changes of THs related genes (trh, tsh, tg, nis, tpo, dio1, dio2, ugt1ab，klf9 and rho), but down-regulated mRNA expression changes of TH receptors (tr α and tr β). Up-regulated tr α and tr β mRNAs by rescue treatment confirmed that both tr α and tr β were involved in the developmental toxicity of BAcAm. In conclusion, our study indicates disruption of THs homeostasis via the thyroid hormone receptors was responsible for the developmental toxicity of BAcAm.

Published in Ecotoxicology and Environmental Safety

ISSN: 0147-6513 (Print); 1090-2414 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Technology: Environmental technology. Sanitary engineering: Environmental pollution; Geography. Anthropology. Recreation: Environmental sciences
Website: https://www.journals.elsevier.com/ecotoxicology-and-environmental-safety

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