The Scientific World Journal (Jan 2002)

Separation of Human Monocytes from a Leukocyte-Rich Plasma

  • John Graham

DOI
https://doi.org/10.1100/tsw.2002.843
Journal volume & issue
Vol. 2
pp. 1646 – 1649

Abstract

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Human peripheral blood monocytes are isolated by flotation from a dense leukocyte-rich plasma (LRP) through two lower-density barriers prepared from OptiPrep™. The separation from lymphocytes depends on the more rapid rate of flotation of the monocytes because of their slightly lower density and larger size. The method works optimally only with fresh (within 2 h of drawing) EDTA-anticoagulated blood.