Tobacco as a model plant (owing to certain features) is extensively used in molecular research with the aims to understand the fundamental plant and pathogen interactions. Fire blight bacterial disease of tobacco (Pseudomonas syringae pv. tabaci (Pst)) causes considerable damage to tobacco worldwide. Induction of genes associated with virulence that play a role in resistance to diseases can be effective in reducing the severity of disease. The evidence suggests that plant hormones are effective in gene expression in plants and increase the production of secondary metabolites. In addition, they stimulate the immune system of plant through the activation of transcription of genes linked with plant defense mechanisms that regulate the induced resistance. In this study, the expression of PR1, PR5, Pal, Catalase and WRKY12 in tobacco plants treated with salicylic acid at a concentration of 3 mM and tobacco plant treated with jasmonic acid at a concentration of 0.5 mM and also control plant in times 0, 12, 24, 48 and 72 after injection of Pseudomonas syrigae pv. tabaci was investigated by Quantitative Real-time PCR (QRT-PCR) technique. The results showed that jasmonic acid and particularly salicylic acid treatments caused significant changes in the expression of these genes after bacterial injection.