Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses

Brijesh B. Karakkat; Kurt Hockemeyer; Margot Franchett; Megan Olson; Cortney Mullenberg; Paul L. Koch

Data in Brief (Oct 2018)

Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses

Brijesh B. Karakkat,
Kurt Hockemeyer,
Margot Franchett,
Megan Olson,
Cortney Mullenberg,
Paul L. Koch

Affiliations

Brijesh B. Karakkat: Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, USA
Kurt Hockemeyer: Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, USA
Margot Franchett: Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, USA
Megan Olson: Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, USA
Cortney Mullenberg: Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, USA
Paul L. Koch: Corresponding author.; Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, USA

Journal volume & issue: Vol. 20
pp. 471 – 479

Abstract

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Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis poae and Ophiosphaerella korrae, “Detection of root-infecting fungi on cool-season turfgrasses using loop-mediated isothermal amplification and recombinase polymerase amplification” (Karakkat et al., 2018) [1]. The data provided here describe the information for designing primers and probes for LAMP and RPA, how specific they are for each of the three fungi, and the evaluation of RPA on field samples. Keywords: Turfgrass, LAMP, RPA, Gaeumannomyces avenae, Ophiosphaerella korrae, Magnaporthiopsis poae

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

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