Heliyon (Aug 2024)
Effect of cadmium toxicity on growth, physiochemical parameters and antioxidant system of castor seedlings
Abstract
The research was aimed to determine the potential impact of cadmium contamination on Ricinus communis. The glucose-6-phosphate dehydrogenase (G6PDH) activity in the root was highest when exposed to 0.2 mM of Cd, with an increase of 15.63 % and 14.48 % at 0 and 24 h, respectively, compared to its control. However, citrate synthase (CS) activity declined in leaves, in contrast, to root, i.e., 12.22 % at 48 h of Cd stress. Isocitrate dehydrogenase (ICDH) activity was maximum in leaves at 0.2 mM of Cd at 0 and 24 h, i.e., 12.36 % and 13.08 % respectively, and later decreased in activity was seen in roots and leaves as the Cd stress increased. Moreover, the level of malate dehydrogenase (MDH) declined in leaves as the Cd level increased, while activity increased in roots at 0.4 mM of Cd i.e., 17.21 %, 17.52 %, and 10.53 % at 0, 24, and 48 h respectively. The important metabolite, glutathione level in the roots of SKP 84 was higher than in the leaf extract. A decline in biomass of up to 28.70 % and 30.91 % and plant length of up to 20.80 % and 26.10 % in shoot and roots, respectively, tolerance index was maximum at 0.2 mM, i.e., 98.62 % was seen. The leaves had 35.40 % catalase (CAT) activity, while the roots had 78.26 % guaiacol peroxidase (GPX) activity at 0.6 mM of Cd. At 0.2 mM of Cd, the maximum activity of ascorbate peroxidase (APX) was observed, with 67.32 % and 62.85 % activity in roots and leaves respectively. However, a reduction in the SOD activity was seen as the Cd stress increased. Increased Cd levels decreased chlorophyll but increased MDA and proline content in leaves at 0.8 mM of Cd, i.e., 82.92 % and 21.7 %, respectively. It indicated that R. communis SKP 84, a fusarium wilt resistance line, is also tolerant to Cd and can be used for phytoremediation in Cd-contaminated areas.
