Warasan Witthayasat Lae Theknoloyi Mahawitthayalai Mahasarakham (Dec 2019)
Effects of Nelumbo nucifera Flower Extracton GK/Jcl Rats
Abstract
The aims of this research were toanalyze the chemical componentsand to determine the biological activities of Nelumbo nucifera flower extract onantioxidant activity, hypoglycemic activity, blood chemistry and serum insulin levels in GK/ Jcl rats.The N. nuciferaflower extractwas prepared bymacerating N. nucifera flower powder in 95% ethanol. The chemincalcomponents of the extract were analysed using GC-MS.The antioxidant activity was tested using DPPH assay. To determine the hypoglycemic activity of the extract, the rats were equally divided into 5 groups with 6 rats in each; groups 1: controlrats received distilled water, group 2: rats received 0.25 mg/kgGlibenclamide, groups 3, 4 and 5:rats received 100, 250 and 500 mg/kg N. nucifera flower extract, respectively, Blood glucose levels were recordedon day 1, 4, and 8. Onday 8,the blood was collected bycardiac puncture andserum was separated foranalysis of blood chemistry andserum insulin levels.The results revealed that 87 chemical components were found intheextract with 5 maincompounds; Nonacosan-10-ol (26.89 %),n-Hexadecanoic acid (14.51 %),9,12-Octadecadienoic acid (Z,Z)- (9.47 %), 9, 12, 15-Octadecatrienoic acid, (Z,Z,Z)- (6.74 %)and 6a-beta-Aporphine, 1, 2-dimethoxy- (3.02 %), and 82 trace components. At a concentration of 400 µg/ml, the extract showeda higher antioxidant activity than BHTwith a percentage radical scavenging of 87.25 ± 1.05% and 49.57 ± 1.22%, respectively.The extract at doses of 100, 250 and 500 mg/kglowered the blood glucose levelsof the ratsin group 3,group 4and group 5 closely to those in group 2. However, theseruminsulin levels of all experimentalgroups and the control group were not significantly different. The results of this study indicate that N. nucifera flower extractpossesses antioxidant and hypoglycemic activity.The underlying mechanism of hypoglycemic activity is partially due to the active compounds found in the extract excluding the responsibility of serum insulin. N. nucifera flower extract is feasiblefor treating people withhigh blood glucose levels or at an initial stage of diabetes.
