Tumor-infiltrating lymphocytes contain a higher proportion of FOXP3+ T lymphocytes in cervical cancer

Abstract

The subpopulations and functions of tumor-infiltrating lymphocytes (TILs) from cervical cancer (CC) are altered. Dysfunction of TIL could be partially because of the inhibition by regulatory T (Treg) cells. FOXP3 is the control gene for the Treg cells. Methods: We investigated the distribution of TILs and FOXP3+ cells in CC (n = 10) and cervical intraepithelial neoplasia (n = 8) tissues. Double-immunofluorescence and confocal-based image quantitative microscopic analysis were used to calculate the number of cluster of differentiation (CD)4+CD25+FOXP3+ Treg cells around the tumor cells. Results: The CD4+CD25+FOXP3+ phenotype of Treg cells was accumulated around the tumor cells. CC contains a significantly higher proportion of the FOXP3+ T cells than in cervical intraepithelial neoplasia (p < 0.001). Moreover, CC with lymph node metastasis has a higher proportion of the FOXP3+ T cells than that without lymph node metastasis (p < 0.05). Conclusion: The increased accumulation of Treg cells suggests that Treg cells are important in the immunopathogenesis of CC.

Keywords

• cervical cancer
• cervical intraepithelial neoplasm
• FOXP3
• Treg cells