Frontiers in Physiology (Feb 2021)
The Study of Angptl4-Modulated Podocyte Injury in IgA Nephropathy
- Sha Jia,
- Sha Jia,
- Sha Jia,
- Sha Jia,
- Sha Jia,
- Sha Jia,
- Xiaofeng Peng,
- Xiaofeng Peng,
- Xiaofeng Peng,
- Xiaofeng Peng,
- Xiaofeng Peng,
- Ludan Liang,
- Ludan Liang,
- Ludan Liang,
- Ludan Liang,
- Ludan Liang,
- Ying Zhang,
- Ying Zhang,
- Ying Zhang,
- Ying Zhang,
- Ying Zhang,
- Meng Li,
- Meng Li,
- Meng Li,
- Meng Li,
- Meng Li,
- Qin Zhou,
- Qin Zhou,
- Qin Zhou,
- Qin Zhou,
- Qin Zhou,
- Xiujin Shen,
- Xiujin Shen,
- Xiujin Shen,
- Xiujin Shen,
- Xiujin Shen,
- Yucheng Wang,
- Yucheng Wang,
- Yucheng Wang,
- Yucheng Wang,
- Yucheng Wang,
- Cuili Wang,
- Cuili Wang,
- Cuili Wang,
- Cuili Wang,
- Cuili Wang,
- Shi Feng,
- Shi Feng,
- Shi Feng,
- Shi Feng,
- Shi Feng,
- Jianghua Chen,
- Jianghua Chen,
- Jianghua Chen,
- Jianghua Chen,
- Jianghua Chen,
- Pingping Ren,
- Pingping Ren,
- Pingping Ren,
- Pingping Ren,
- Pingping Ren,
- Hong Jiang,
- Hong Jiang,
- Hong Jiang,
- Hong Jiang,
- Hong Jiang
Affiliations
- Sha Jia
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Sha Jia
- Key Laboratory of Nephropathy, Hangzhou, China
- Sha Jia
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Sha Jia
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Sha Jia
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Sha Jia
- Dongyang Women & Children Hospital, Dongyang, China
- Xiaofeng Peng
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Xiaofeng Peng
- Key Laboratory of Nephropathy, Hangzhou, China
- Xiaofeng Peng
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Xiaofeng Peng
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Xiaofeng Peng
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Ludan Liang
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Ludan Liang
- Key Laboratory of Nephropathy, Hangzhou, China
- Ludan Liang
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Ludan Liang
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Ludan Liang
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Ying Zhang
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Ying Zhang
- Key Laboratory of Nephropathy, Hangzhou, China
- Ying Zhang
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Ying Zhang
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Ying Zhang
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Meng Li
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Meng Li
- Key Laboratory of Nephropathy, Hangzhou, China
- Meng Li
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Meng Li
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Meng Li
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Qin Zhou
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Qin Zhou
- Key Laboratory of Nephropathy, Hangzhou, China
- Qin Zhou
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Qin Zhou
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Qin Zhou
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Xiujin Shen
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Xiujin Shen
- Key Laboratory of Nephropathy, Hangzhou, China
- Xiujin Shen
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Xiujin Shen
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Xiujin Shen
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Yucheng Wang
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Yucheng Wang
- Key Laboratory of Nephropathy, Hangzhou, China
- Yucheng Wang
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Yucheng Wang
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Yucheng Wang
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Cuili Wang
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Cuili Wang
- Key Laboratory of Nephropathy, Hangzhou, China
- Cuili Wang
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Cuili Wang
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Cuili Wang
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Shi Feng
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Shi Feng
- Key Laboratory of Nephropathy, Hangzhou, China
- Shi Feng
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Shi Feng
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Shi Feng
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Jianghua Chen
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Jianghua Chen
- Key Laboratory of Nephropathy, Hangzhou, China
- Jianghua Chen
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Jianghua Chen
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Jianghua Chen
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Pingping Ren
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Pingping Ren
- Key Laboratory of Nephropathy, Hangzhou, China
- Pingping Ren
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Pingping Ren
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Pingping Ren
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- Hong Jiang
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Hong Jiang
- Key Laboratory of Nephropathy, Hangzhou, China
- Hong Jiang
- Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China
- Hong Jiang
- Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China
- Hong Jiang
- Institute of Nephropathy, Zhejiang University, Hangzhou, China
- DOI
- https://doi.org/10.3389/fphys.2020.575722
- Journal volume & issue
-
Vol. 11
Abstract
BackgroundIncreasing evidence shows that Angptl4 affects proteinuria in podocytes injured kidney disease, however, whether there is a relationship between Angptl4 and IgA nephropathy (IgAN) has not been studied yet.MethodsPlasma and urine samples were obtained from 71 patients with IgAN and 61 healthy controls. Glomeruli from six renal biopsy specimens (three IgAN patients and three healthy controls) were separated by RNA-Seq. Differentially expressed genes (DEGs) related to podocytes and Angptl4 between IgAN patients and healthy controls were performed using the Limma package. Gene set enrichment analysis was used to determine whether there was a statistically significant difference between the two groups. STRING was used to create a protein-protein interaction network of DEGs. Association analysis between Angptl4 levels and clinical features of IgAN was performed.ResultsThirty-three podocyte-related and twenty-three Angpt4-related DEGs were found between IgAN patients and healthy controls. By overlapping the genes, FOS and G6PC were found to be upregulated in IgAN patients, while MMP9 was downregulated in IgAN patients. Plasma and urine Angptl4 levels were closely related to the degree of podocyte injury and urine protein, but not to the protein-creatine ratio.ConclusionOur findings show that Angptl4 levels in plasma and urine are related to podocyte damage and, therefore, may be a promising tool for assessing the severity of IgAN patients to identify and reverse the progression to ESRD.
Keywords
