Журнал микробиологии, эпидемиологии и иммунобиологии (Aug 2019)

Analysis of prevalence of HIV-1 primary resistance to antiretroviral drugs in the territory of Moscow and Moscow region

  • M. N. Nosik,
  • K. A. Ryzhov,
  • A. V. Kravchenko,
  • S. E. Sevostyanihin,
  • U. A. Kuimova,
  • А. B. Potapova,
  • A. L. Sobkin

DOI
https://doi.org/10.36233/0372-9311-2019-3-27-31
Journal volume & issue
Vol. 0, no. 3
pp. 27 – 31

Abstract

Read online

Aim. To analyze the level of HIV drug resistance among primary patients (who did not receive antiretroviral therapy (ART)) living in the Moscow region, where access to the antiretroviral therapy is quite high.Material and methods. Blood samples from 527 drug-nanve patients who live in the Moscow (n=279) and in the Moscow region (n=248) were examined for the period 2008-2015 years. For detecting and analyzing possible mutations of resistance virus genome nucleotide sequence region was studied using the test systems ViroseqTMHIV-1 Genotyping System and StanfordnHIV Drug Resistance database (http://cpr.stanford.edu/cpr.cgi).Results. It was found that the level of transmission of HIV-1 resistant strains among na^e-drug HIV-positive patients is still quite low, that is 2%. However, the percentage of polymorphism mutations and secondary substitutions which in combination with other resistance mutations in the reverse transcriptase gene can reduce the virus sensitivity to antiretroviral drugs (ARVs) is quite high — 34.5%.Conlusion. The level of primary resistance among drug-nanve HIV-infected persons does not exceed 5%. However, given the wide access to ART and the emergence in recent years of ambiguous mutations and deletions in the reverse transcriptase/ protease gene that could potentially lead to ARVs resistance, it is obvious that continuous monitoring of the circulation of resistant strains is necessary.

Keywords