Nature Communications (Oct 2020)
A versatile soluble siglec scaffold for sensitive and quantitative detection of glycan ligands
- Emily Rodrigues,
- Jaesoo Jung,
- Heajin Park,
- Caleb Loo,
- Sepideh Soukhtehzari,
- Elena N. Kitova,
- Fahima Mozaneh,
- Gour Daskhan,
- Edward N. Schmidt,
- Vivian Aghanya,
- Susmita Sarkar,
- Laura Streith,
- Chris D. St. Laurent,
- Linh Nguyen,
- Jean-Philippe Julien,
- Lori J. West,
- Karla C. Williams,
- John S. Klassen,
- Matthew S. Macauley
Affiliations
- Emily Rodrigues
- Department of Chemistry, University of Alberta
- Jaesoo Jung
- Department of Chemistry, University of Alberta
- Heajin Park
- Department of Chemistry, University of Alberta
- Caleb Loo
- Department of Chemistry, University of Alberta
- Sepideh Soukhtehzari
- Department of Pharmaceutical Sciences, University of British Columbia
- Elena N. Kitova
- Department of Chemistry, University of Alberta
- Fahima Mozaneh
- Department of Chemistry, University of Alberta
- Gour Daskhan
- Department of Chemistry, University of Alberta
- Edward N. Schmidt
- Department of Chemistry, University of Alberta
- Vivian Aghanya
- Department of Chemistry, University of Alberta
- Susmita Sarkar
- Department of Chemistry, University of Alberta
- Laura Streith
- Department of Medical Microbiology and Immunology, University of Alberta
- Chris D. St. Laurent
- Department of Chemistry, University of Alberta
- Linh Nguyen
- Department of Chemistry, University of Alberta
- Jean-Philippe Julien
- Departments of Biochemistry and Immunology, University of Toronto
- Lori J. West
- Department of Medical Microbiology and Immunology, University of Alberta
- Karla C. Williams
- Department of Pharmaceutical Sciences, University of British Columbia
- John S. Klassen
- Department of Chemistry, University of Alberta
- Matthew S. Macauley
- Department of Chemistry, University of Alberta
- DOI
- https://doi.org/10.1038/s41467-020-18907-6
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 13
Abstract
Sialic acid-binding immunoglobulin-type lectins (Siglecs) are a family of immunomodulatory receptors expressed on cells of the hematopoietic lineage. Here the authors demonstrate an approach for the identification of the glycan ligands of Siglecs, which is also applicable to other families of glycan-binding proteins.
