The Anti-Inflammatory Effects of Iberin on TNF-α-Stimulated Human Oral Epithelial Cells: In Vitro Research
Yoshitaka Hosokawa,
Ikuko Hosokawa,
Masahiro Shimoyama,
Ayumi Fujii,
Juri Sato,
Kimitake Kadena,
Kazumi Ozaki,
Keiichi Hosaka
Affiliations
Yoshitaka Hosokawa
Department of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Tokushima, Japan
Ikuko Hosokawa
Department of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Tokushima, Japan
Masahiro Shimoyama
Department of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Tokushima, Japan
Ayumi Fujii
Department of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Tokushima, Japan
Juri Sato
Department of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Tokushima, Japan
Kimitake Kadena
Department of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Tokushima, Japan
Kazumi Ozaki
Department of Oral Health Care Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Tokushima, Japan
Keiichi Hosaka
Department of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Tokushima, Japan
Iberin is a bioactive chemical found in cruciferous plants that has been demonstrated to have anticancer properties. However, there have been no reports on its effects on periodontal resident cells, and many questions remain unanswered. The aim of this study was to examine whether iberin had anti-inflammatory effects on human oral epithelial cells, including influences on signal transduction pathway activation in TNF-α-στιμυλατεd χελλσ. Iberin inhibited the production of interleukin (IL)-6 and C-X-C motif chemokine ligand 10 (CXCL10), as well as the expression of vascular cell adhesion molecule (VCAM)-1, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 in tumor necrosis factor (TNF)-α-stimulated TR146 cells, a human oral epithelial cell line. Moreover, iberin administration increased the expression of antioxidant signaling pathways, such as Heme Oxygenase (HO)-1 and NAD(P)H quinone dehydrogenase 1 (NQO1). Furthermore, we found that iberin could inhibit the activation of the nuclear factor (NF)-κB, signal transducer and activator of transcription (STAT)3, and p70S6 kinase (p70S6K)-S6 ribosomal protein (S6) pathways in TNF-α-stimulated TR146 cells. In conclusion, iberin reduced inflammatory mediator expression in human oral epithelial cells by preventing the activation of particular signal transduction pathways.
