De novo design and directed folding of disulfide-bridged peptide heterodimers

Sicong Yao; Adam Moyer; Yiwu Zheng; Yang Shen; Xiaoting Meng; Chong Yuan; Yibing Zhao; Hongwei Yao; David Baker; Chuanliu Wu

doi:10.1038/s41467-022-29210-x

Nature Communications (Mar 2022)

De novo design and directed folding of disulfide-bridged peptide heterodimers

Sicong Yao,
Adam Moyer,
Yiwu Zheng,
Yang Shen,
Xiaoting Meng,
Chong Yuan,
Yibing Zhao,
Hongwei Yao,
David Baker,
Chuanliu Wu

Affiliations

Sicong Yao: Department of Chemistry, College of Chemistry and Chemical Engineering, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University
Adam Moyer: Department of Biochemistry and Institute for Protein Design, University of Washington
Yiwu Zheng: Department of Chemistry, College of Chemistry and Chemical Engineering, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University
Yang Shen: Department of Chemistry, College of Chemistry and Chemical Engineering, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University
Xiaoting Meng: Department of Chemistry, College of Chemistry and Chemical Engineering, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University
Chong Yuan: Institute of Molecular Enzymology, School of Biology and Basic Medical Sciences, Soochow University
Yibing Zhao: Department of Chemistry, College of Chemistry and Chemical Engineering, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University
Hongwei Yao: Institute of Molecular Enzymology, School of Biology and Basic Medical Sciences, Soochow University
David Baker: Department of Biochemistry and Institute for Protein Design, University of Washington
Chuanliu Wu: Department of Chemistry, College of Chemistry and Chemical Engineering, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University

DOI: https://doi.org/10.1038/s41467-022-29210-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

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Peptide heterodimers are prevalent in nature, which are not only functional macromolecules but molecular tools for chemical and synthetic biology. Here the authors report de novo design and directed folding of peptide heterodimers crosslinked through multiple disulfide bonds, which can be explored as chemical tools for orthogonal labeling of proteins and preparing protein hybrids.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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