Building an International One Health Strain Level Database to Characterise the Epidemiology of AMR Threats: ESBL—AmpC Producing <i>E. coli</i> as An Example—Challenges and Perspectives
Sara Perestrelo,
Ana Amaro,
Michael S. M. Brouwer,
Lurdes Clemente,
Ana Sofia Ribeiro Duarte,
Annemarie Kaesbohrer,
Renata Karpíšková,
Vicente Lopez-Chavarrias,
Dearbháile Morris,
Deirdre Prendergast,
Angela Pista,
Leonor Silveira,
Magdalena Skarżyńska,
Rosemarie Slowey,
Kees T. Veldman,
Magdalena Zając,
Catherine Burgess,
Julio Alvarez
Affiliations
Sara Perestrelo
Department of Biological Safety, German Federal Institute for Risk Assessment, 10589 Berlin, Germany
Ana Amaro
Laboratory of Bacteriology and Micology, National Institute of Agrarian and Veterinary Research, National Reference for Animal Health, 2780-157 Oeiras, Portugal
Michael S. M. Brouwer
Department of Bacteriology, Host Pathogen Interaction & Diagnostics, Wageningen Bioveterinary Research, Part of Wageningen University & Research, 8221 Lelystad, The Netherlands
Lurdes Clemente
Laboratory of Bacteriology and Micology, National Institute of Agrarian and Veterinary Research, National Reference for Animal Health, 2780-157 Oeiras, Portugal
Ana Sofia Ribeiro Duarte
National Food Institute, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
Annemarie Kaesbohrer
Department of Biological Safety, German Federal Institute for Risk Assessment, 10589 Berlin, Germany
Renata Karpíšková
Department of Public Health, Medical Faculty, Masaryk University, 625 000 Brno, Czech Republic
Vicente Lopez-Chavarrias
VISAVET Health Surveillance Centre, Universidad Complutense, 28040 Madrid, Spain
Dearbháile Morris
Antimicrobial Resistance and Microbial Ecology Group, School of Medicine, University of Galway, H91 TK33 Galway, Ireland
Deirdre Prendergast
Backweston Laboratory Campus, Department of Agriculture, Food and the Marine, W23 X3PH Celbridge, Ireland
Angela Pista
National Reference Laboratory for Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal
Leonor Silveira
National Reference Laboratory for Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal
Magdalena Skarżyńska
Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
Rosemarie Slowey
Backweston Laboratory Campus, Department of Agriculture, Food and the Marine, W23 X3PH Celbridge, Ireland
Kees T. Veldman
Department of Bacteriology, Host Pathogen Interaction & Diagnostics, Wageningen Bioveterinary Research, Part of Wageningen University & Research, 8221 Lelystad, The Netherlands
Magdalena Zając
Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
Catherine Burgess
Food Safety Department, Teagasc Food Research Centre Ashtown, D15 DY05 Dublin, Ireland
Julio Alvarez
VISAVET Health Surveillance Centre, Universidad Complutense, 28040 Madrid, Spain
Antimicrobial resistance (AMR) is one of the top public health threats nowadays. Among the most important AMR pathogens, Escherichia coli resistant to extended spectrum cephalosporins (ESC-EC) is a perfect example of the One Health problem due to its global distribution in animal, human, and environmental sources and its resistant phenotype, derived from the carriage of plasmid-borne extended-spectrum and AmpC β-lactamases, which limits the choice of effective antimicrobial therapies. The epidemiology of ESC-EC infection is complex as a result of the multiple possible sources involved in its transmission, and its study would require databases ideally comprising information from animal (livestock, companion, wildlife), human, and environmental sources. Here, we present the steps taken to assemble a database with phenotypic and genetic information on 10,763 ESC-EC isolates retrieved from multiple sources provided by 13 partners located in eight European countries, in the frame of the DiSCoVeR Joint Research project funded by the One Health European Joint Programme (OH-EJP), along with its strengths and limitations. This database represents a first step to help in the assessment of different geographical and temporal trends and transmission dynamics in animals and humans. The work performed highlights aspects that should be considered in future international efforts, such as the one presented here.
