Film polysaccharide matrices for immobilization of hydrophilic fluorescence probes

Abstract

The selection of a suitable matrix for immobilizing a reagent is one of the key issues in the development of a luminescent chemosensor. Various polysaccharide matrices from cellulose, cellulose nitrate, cellulose diacetate, chitosan and its acrylic copolymers have been investigated as a platform for immobilizing fluorescent probes (eosin Y and tripaflavin) for using in optical chemosensors. The parameters of the dyes sorption from aqueous solutions on the matrices were determined. Matrices with high signals of solid-surface fluorescence of the probes were revealed. A film matrix made of cellulose diacetate was shown to be the most promising sorbent for tripaflavine. A composite matrix based on chitosan and cellulose and a matrix made of chitosan modified with formic acid were the best for eosin immobilization. The developed sensitive elements (matrix ​+ ​dye) can be used to design optical chemosensors.

Keywords

• Polysaccharide matrices
• Cellulose diacetate
• Chitosan
• Solid-surface fluorescence
• Fluorescence probes
• Eosin