European Oral Research (Jan 2019)
In vitro antimicrobial activity of different electrochemicallyactivated solutions on enterococcus faecalis
Abstract
PurposeThe aim of this in vitro study was to assess and compare the antimicrobial efficacyof different electrochemically-activated solutions (ECA) and contemporaryirrigants, in root canals infected with Enterococcus faecalis, used with or withoutEndoActivator (EA).Materials and methodsA hundred single-rooted human teeth were prepared. Ninety of the rootsegments were infected with E. faecalis for four weeks, and divided into eighttest groups (n = 10) (four with and four without EA sonication) and a positivecontrol (n = 10). The irrigants tested were electrochemically-activated solutionsproduced by the Medilox® (ECA-MX) and Envirolyte® devices (ECA-EN), 2%CHX and 2.5% NaOCl. The root specimens were irrigated with 5 mL of the testsolution, with additional sonic agitation applied to the EA groups. The dentinesamples that were obtained from the walls were cultured, and the antibacterialefficacy was evaluated by counting the colony-forming units.ResultsThe ECA-EN, 2.5% NaOCl and 2% CHX were more effective than the ECA-MX (p <0.05) with the addition of EA sonication, showing no statistical difference in theelimination of E. faecalis.ConclusionThe ECA-EN shows potential as an endodontic irrigant, while EA usage gives nobenefit in reducing bacteria from root canals.
