Spectrometric method for simultaneous quantification of nadifloxacin and salicylic acid in a novel topical dosage form

Abstract

Acne vulgaris is a prevalent skin disorder that affects many individuals. In the search for effective acne therapy, researchers have found that a combination of Nadifloxacin and Salicylic Acid may produce a synergistic effect. To further investigate this, a study was conducted to devise a spectrophotometric method for simultaneously measuring the concentrations of Nadifloxacin and Salicylic Acid in a novel topical formulation. Simultaneous Estimation Method was employed using methanol as the solvent. Nadifloxacin exhibited an absorbance maximum of 291 nm, while Salicylic Acid showed an absorbance maximum of 297 nm in methanol. Linearity experiments were conducted to determine the concentration ranges for both drugs, which were found to be 2–12 ppm for Nadifloxacin and 5–30 ppm for Salicylic Acid. The recovery results of Nadifloxacin ranged from 82 % to 95 %, while those of Salicylic Acid ranged from 97 % to 110 %. These results indicate the accuracy and reliability of the method. This method was further validated in accordance with the guidelines provided by the International Council for Harmonization (ICH) of Technical Requirements for Human Use. The proposed method has demonstrated its applicability for the simultaneous and quantitative assessment of Nadifloxacin and Salicylic Acid in various dosage forms. This development is significant as it provides a reliable and efficient means of measuring the concentrations of these two drugs in acne therapy formulations.

Keywords

• Simultaneous estimation method
• UV-visible spectrophotometer
• Nadifloxacin
• Salicylic Acid
• Acne-vulgaris