Acute and Subacute Toxicity and Cytotoxicity of <i>Opuntia Dillenii</i> (Ker-Gawl) Haw. Seed Oil and Its Impact on the Isolated Rat Diaphragm Glucose Absorption
Mohamed Bouhrim,
Salima Boutahiri,
Loubna Kharchoufa,
Hamza Mechchate,
Omkulthom Mohamed Al Kamaly,
Ali Berraaouan,
Bruno Eto,
Abderrahim Ziyyat,
Hassane Mekhfi,
Abdelkhaleq Legssyer,
Mohammed Aziz,
Mohamed Bnouham
Affiliations
Mohamed Bouhrim
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences Mohammed First University, Oujda B.P. 717, Morocco
Salima Boutahiri
Univ. Lille, University of Liège, University of Picardy Jules Verne, JUNIA, UMRT 1158 BioEcoAgro, Specialized Metabolites of Plant Origin, F-59000 Lille, France
Loubna Kharchoufa
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences Mohammed First University, Oujda B.P. 717, Morocco
Hamza Mechchate
Laboratory of biotechnology, Environment, Agri-Food, and Health (LBEAS), Faculty of Sciences, Dhar El Mahraz, Sidi Mohamed Ben Abdellah University (USMBA), Fez B.P. 1796, Morocco
Omkulthom Mohamed Al Kamaly
Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh 11564, Saudi Arabia
Ali Berraaouan
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences Mohammed First University, Oujda B.P. 717, Morocco
Bruno Eto
Laboratories—TBC, Faculty of Pharmaceutical and Biological Sciences, B.P. 83 Lille, France
Abderrahim Ziyyat
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences Mohammed First University, Oujda B.P. 717, Morocco
Hassane Mekhfi
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences Mohammed First University, Oujda B.P. 717, Morocco
Abdelkhaleq Legssyer
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences Mohammed First University, Oujda B.P. 717, Morocco
Mohammed Aziz
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences Mohammed First University, Oujda B.P. 717, Morocco
Mohamed Bnouham
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences Mohammed First University, Oujda B.P. 717, Morocco
This study aims to assess the safety of the Opuntia dillenii (Ker-Gawl) haw. seed oil (ODSO) and its effect on the glucose absorption activity of the isolated rat hemidiaphragm. This oil’s safety was studied by exploring its acute (doses 1, 3, 5, and 7 mL/kg) and subacute (doses 1 and 2 mL/kg) toxicities in albino mice and Wistar rats, respectively. The safety of the ODSO was also assessed by studying its effect on the HepG2 cell viability in vitro. The effect of ODSO, or combined with the insulin, on the glucose absorption activity of isolated rat hemidiaphragm was evaluated at the dose 1 g/L in vitro. The results demonstrated the safety of ODSO. Indeed, this study showed that this oil does not produce any mortality or signs of toxicity after the single-dose administration in mice. Additionally, the daily intake of the ODSO during four weeks does not induce a significant variation in the biochemical parameters and body weight of rats compared with the control group. Besides, the cell viability of HepG2 did not change in the presence of ODSO. On the other hand, the ODSO increased the glucose absorption activity of the isolated rat hemidiaphragm, and this activity was significantly enhanced when combined with insulin. This study confirms, on one side, the safety of this oil and its efficacy and, on the other side, encourages its potential use as a complement to treat diabetes.
