Cell Positioning Culture Chip Based on Functional Micro Topological Substrate

Abstract

There are two main categories of cell positioning culture chips:chemical modification of surfaces and physical structure methods. The chemical methods have the advantage of providing a strong binding force, but the instability of chemical materials limits its application. The physical methods have the advantage of good stability for cell positioning, while the nonspecificity of physical structure weakens the ability to regulate cells. Here, we propose a functional micro topological chip. The chip is composed of micro-platform and micro-post array with different adhesion characteristics. At the same time, the chip is prepared by a polymer material with good biocompatibility and high chemical stability; therefore, it has the advantages of strong cell constraint ability and good localization stability. The result of chip performance verification shows that the cell localization rate of the functional micro topological chip is up to 90%, and the chip structure and dimension stability are good. The study provides an important supporting tool for the research of stem cells and their differentiation that need long-term cell culture.

Keywords

• cell positioning culture chip
• functional micro topology
• micro pattern