Data set of the proteome of fresh and frozen thawed stallion spermatozoa
Francisco E. Martín-Cano,
Gemma Gaitskell-Phillips,
José M. Ortiz-Rodríguez,
Antonio Silva,
Cruz Gil,
Cristina Ortega-Ferrusola,
Fernando J. Peña
Affiliations
Francisco E. Martín-Cano
Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain
Gemma Gaitskell-Phillips
Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain
José M. Ortiz-Rodríguez
Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain
Antonio Silva
SIPA University of Extremadura, Cáceres, Spain
Cruz Gil
Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain
Cristina Ortega-Ferrusola
Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain
Fernando J. Peña
Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain; Corresponding author at: Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain
This paper provides the dataset of proteins of stallion ejaculates before and after cryopreservation. The data report the analysis and identification of stallion sperm proteins obtained from the same ejaculates and split in two subsamples. The first aliquot consisted on fresh spermatozoa and the second aliquot was frozen and thawed spermatozoa. Samples were analyzed using a UHPLC/MS/MS system consisting of an Agilent 1290 infinity series UHPLC coupled to an Agilent 6550 Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). We provide a data set of 2226 different proteins, with 2180 aligned to the equine proteome database. The data can be used to identify potential targets to be explored to improve techniques for cryopreservation of spermatozoa. This data article refers to the article “Proteomic profiling of stallion spermatozoa suggests changes in sperm metabolism and compromised redox regulation after cryopreservation” (Martín Cano et al.; 2020) [1].
