Influence of targeted inhibition of DNAJC1 gene on apoptosis and proliferation in multiple myeloma cells

Abstract

Objective To determine the effect of DNAJC1 gene on the proliferation and apoptosis of multiple myeloma cell lines and preliminarily investigate its mechanism. Methods DNAJC1-siRNA and its negative control (siRNA) were transfected into human multiple myeloma cell line U266 respectively. The interference efficiency was determined by RT-qPCR and Western blotting, and cell proliferation and apoptotic rate were measured with CCK-8 assay and flow cytometry, respectively. In addition, RT-qPCR and Western blotting were also used to examine the mRNA and protein expression of PTEN in U266 cells. Results The mRNA and protein levels of DNAJC1 were downregulated in the interference group. The proliferation of U266 cells was inhibited significantly in the interference group, with an inhibitory rate of (58.95±8.13)%, apparently higher than that of the negative siRNA group (P < 0.05). The apoptotic rate was (44.95±6.06)% in the interference group, which notably exceeded that of the negative siRNA group and the control group (P < 0.05). The mRNA and protein levels of PTEN were significantly increased in the interference group (P < 0.05). Conclusion Targeted inhibition of DNAJC1 gene can promote the apoptosis and inhibit the proliferation in multiple myeloma cells, which may be mediated by up-regulation of PTEN.

Keywords

• dnajc1 gene
• multiple myeloma
• cell apoptosis
• cell proliferation
• pten