This work presents the results of the fluoride removal in water, through an adsorption process with the use of a natural Mexican zeolite conditioned with calcium, manganese or iron in a fixed bed column system. The XRD and FTIR characterization results demonstrated that the conditioning modifies the surface chemical composition of the zeolite and the SEM/EDS analysis corroborates the increase of the exchanged ions. The conditioning of the zeolite generated an increase in the adsorption capacity of fluorine, the best percentage of removal obtained from fluoride ions was 98%, for a water with a concentration of 10 mg·L−1 with the conditioned zeolite with iron.