Sensors (Oct 2011)

A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I

  • Gang Liu,
  • Lihua Wang,
  • Jingwen Chen,
  • Shuzhen Ren,
  • Dun Pan,
  • Quanwen Liu,
  • Hui Xu,
  • Shuli Gao,
  • Min Ding

DOI
https://doi.org/10.3390/s111110187
Journal volume & issue
Vol. 11, no. 11
pp. 10187 – 10196

Abstract

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We here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+-thymine (T-Hg2+-T) complex. Cys can selectively coordinate with Hg2+ and extract it from the thymine-Hg2+-thymine complex. The hairpin structure dehybridizes and the fluorescence intensity of Sybr Green I (SG) decreases upon addition of Cys because SG efficiently discriminates mercury-specific DNA and mercury-specific DNA/Hg2+ complex. The detection can be finished within 5 min with high sensitivity and selectivity. In addition, we can obtain variable dynamic ranges for Cys by changing the concentration of MSD/Hg2+.

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