PLoS ONE (Jan 2015)

Oligomerization of Baculovirus LEF-11 Is Involved in Viral DNA Replication.

  • Zhan-Qi Dong,
  • Nan Hu,
  • Jun Zhang,
  • Ting-Ting Chen,
  • Ming-Ya Cao,
  • Hai-Qing Li,
  • Xue-Jiao Lei,
  • Peng Chen,
  • Cheng Lu,
  • Min-Hui Pan

DOI
https://doi.org/10.1371/journal.pone.0144930
Journal volume & issue
Vol. 10, no. 12
p. e0144930

Abstract

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We have previously reported that baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) late expression factor 11 (lef-11) is associated with viral DNA replication and have demonstrated that it potentially interacts with itself; however, whether LEF-11 forms oligomers and the impact of LEF-11 oligomerization on viral function have not been substantiated. In this study, we first demonstrated that LEF-11 is capable of forming oligomers. Additionally, a series of analyses using BmNPV LEF-11 truncation mutants indicated that two distinct domains control LEF-11 oligomerization (aa 42-61 and aa 72-101). LEF-11 truncation constructs were inserted into a lef-11-knockout BmNPV bacmid, which was used to demonstrate that truncated LEF-11 lacking either oligomerization domain abrogates viral DNA replication. Finally, site-directed mutagenesis was used to determine that the conserved hydrophobic residues Y58&I59 (representing Y58 and I59), I85 and L88&L89 (representing L88 and L89) are required for LEF-11 oligomerization and viral DNA replication. Collectively, these data indicate that BmNPV LEF-11 oligomerization influences viral DNA replication.