Mediators of Inflammation (Jan 1996)

Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone

  • M. M. Verheggen,
  • H. I. M. de Bont,
  • P. W. C. Adriaansen-Soeting,
  • B. J. A. Goense,
  • C. J. A. M. Tak,
  • H. C. Hoogsteden,
  • P. Th. W. van Hal,
  • M. A. Versnel

DOI
https://doi.org/10.1155/S0962935196000300
Journal volume & issue
Vol. 5, no. 3
pp. 210 – 217

Abstract

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In this study, we investigated the expression of lipocortin I and II (annexin I and I in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-1β, TNF-α or LPS for 24 h did not affect the lipocortin I or II mRNA or protein expression, although PGE2 and 6-keto-PGF1α production was significantly increased. This IL-1β- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction.