Teratogenic effect on bone tissue development in Rattus norvegicus Wistar strain, induced by the presence of arsenic

Abstract

In this study we evaluated the possible teratogenic effects of arsenic (As) in the same concentrations recorded in water Zimapan, Hidalgo, using specimens of Rattus norvegicus Wistar strain, pregnant. Were subjected to varying concentrations of sodium arsenate (1887.9 mg/L, 943.9 mg/L and 0.5 mg/L), concentration, 0.5 mg/L corresponds to the registered Zimapan water, Hidalgo and chromium trioxide (166.0 mg/L) used as positive control and 5% sucrose as a negative control. The specimens were exposed from day 7 through 15 of gestation, and were sacrificed on day 19 of gestation by inhalation of chloroform. We analyzed reproductive harm the mother, as well as alterations and lack of ossification of the bone tissue of the fetus. The highest dose tested (1887.9 mg/L) produced more reproductive harm in mother resorptions caused by preventing the continuation of embryonic development. Concentrations of 0.5 mg/L to 943.9 mg/L, resulted in lack of ossification ribs, sternebrae, metacarpals, phalanges previous metatarsals and phalanges posterior sacral vertebrae.

Keywords

• sodium arsenate
• teratogenic
• reproductive harm
• lack of ossification
• Rattus norvegicus