Sequence analysis of mitochondrial genome of the false and phantom crane-fly Ptychoptera qinggouensis Kang, Yao and Yang, 2013 (Diptera, Ptychopteridae)

Abstract

The genus Ptychoptera Meigen, is the largest genus of the family Ptychopteridae with 78 known species. In this study, we report a nearly complete mitochondrial (mt) genome of this genus, which is a circular molecule of more than 15,028 bp. The mt genome contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a non-coding region. The overall base composition is A (38.1%), T (36.7%), C (14.9%), and G (10.4%), with an AT content of 74.8%. The AT content of N-strand genes (75.7%) is higher than that of the J-strand genes (71.7%). Phylogenetic analysis reveals that the monophyly of Ptychopteridae, Bibiomorpha, Tipulomorpha and Brachycera are strongly supported, and the sister group relationship between Tanyderidae and Ptychopteridae is not supported.

Keywords

• mitochondrial genome
• phylogeny
• ptychoptera
• false and phantom crane-fly