Histological and Morphometrical Studies in Liver Regeneration in Mice

Abstract

Liver regeneration after loss of important cell population is a complex process involving all mature liver cell types. We study the response of hepatocytes in 24h and 48h after partial hepatectomy by microscopic and morphometric analysis. Thirty adult male NMRI, 8 week old mice were used in the study. Liver regeneration after partial hepatectomy (68%) in mice is a complex process that is performed on account differentiated mature hepatocyte proliferation, as evidenced by the presence of nuclei hypertrophy, binucleate cells or polyploidy, and numerous mitotic figures. Morphometrical analysis of cell size showed increases in average dimension of the control compared to the experimental groups. After this study, we can conclude that liver regeneration after partial hepatectomy is marked by the proliferation of liver cell population, while reducing the apoptotic process.

Keywords

• histology
• liver regeneration
• morphometry